WP1_DEVELOPMENT OF PLATFORM CELL FACTORIES
GLYCOSIDE PHOSPHORYLASES AS INDUSTRIAL GLYCOSYLATION BIOCATALYSTS
Biocatalysts are engineered for optimized conversion of the glucose part from sucrose into innovative functional glucosides.
The optimisation concept of CARBAFIN is to engineer flexible cell factories (preferred: Escherichia coli) for high-level expression of coupled phosphorylases to obtain whole-cell glycosylation biocatalysts sufficiently active and robust for use in the production processes of the target glucosides. Focus is put on enzyme engineering, strain optimisation and synthetic biology for balanced co-expression.
ACIB, TUG, UGent
Tom Desmet (UGent)