The CARBAFIN team of acib and Graz, University of Technology has published a paper on continuous bioprocessing of glucosides from sucrose using the enzyme sucrose-phosphorylase. The advantage of this continuous approach is the tight interconnection of production (upstream) and purification (downstream) process steps as well as a stable operation at excellent output parameters over weeks. A crucial part of the technology is the catalyst immobilization by whole cell encapsulation in porous polymer materials. In other words, a kind of a “nanobioreactor” is created to produce the desired glucoside more efficiently.
The process paves the way for an intensified glucoside production at industrial scale and shows considerable replication potential for other glycosylation products derived from sucrose.