Another successful CARBAFIN year comes to an end! We have summarized all of our highlights in 2021 in our third newsletter edition and wish you a happy read!
Enjoy your Christmas holidays with your families and friends, stay healthy and have a good start into 2022!
All the best from the CARBAFIN team.
The CARBAFIN research team from acib has published an article in “Microbial Cell Factories”. It describes the use of a whole cell-based catalyst for the enzymatic production of glucosylglycerol, one of the main CARBAFIN target compounds. Glucosylglycerol has promising applications in cosmetic, food and feed formulations. We consider whole cell biocatalysis to be an excellent choice, because the enzyme is accumulated in large amounts inside the cell. That offers a highly active and ready-to-use sucrose phosphorylase, which is one of the key biocatalysts of the CARBAFIN glycosylation technology.
Especially, the sucrose phosphorylase from the bacterium Leuconostoc mesenteroides turned out to be a promising candidate for a whole cell-based catalyst formulation in Escherichia coli. These findings have been published and are available here.
EFIB2021 is over and about 50 participants have joined the CARBAFIN pre-conference workshop “The future of CARBAFIN’s glycosylation technology in industrial production processes”.
At first, participants were introduced to the project, its objectives, concepts and key results by project manager Christiane Luley (acib). Then, industrial representatives Thomas Häßler (Pfeifer & Langen), Steven Koenig (bitop) and Stefan Krawielitzki (AVA Biochem) showed different use cases of the glycosylation technology in food, cosmetics or bulk chemistry applications. But the various application fields might be even broader, which was shown by the presentations of external industry partners, Michael Merz (Nestlé), Ronny Vercauteren (Cargill) and Regis Marchand (SEPPIC).
To discuss the replication potential of CARBAFIN and future collaborations in further detail, participants came together in a world café discussion and concentrated on three topics:
- Replication potential of CARBAFIN’s glycosylation technology
- Requirements of life-cycle-assessments for process validations
- Expectations of collaborations in European project consortia
Get a summary of the world café discussion here.
With respect to circular economy and the use of renewable feedstocks, the European project CARBAFIN has developed a radically new value chain for the utilization of sucrose from sugar beet biomass. Functional glycosides (eg for prebiotics or cosmetics) or platform chemicals (eg for bioplastics, biofuels or biopolymers) are important product examples and open new and economically relevant perspectives for the European sugar industry to several industrial sectors. Biocatalytic production processes were elevated to an industrial level using an integrated process design approach accompanied by environmental and economic evaluation.
In this satellite stakeholder workshop we would like to discuss the replication potential of this glycosylation technology and explore the opportunities thereof in different industries: Representatives from the cosmetic, food, feed, pharma or (bulk) chemical industry as well as scientists are welcome to identify new solutions for a bio-based re-industrialization in Europe and to discuss new ways of valorization.
Learn about CARBAFIN’s outcomes and results, find new collaboration partners and take part in our joint expert’s discussion in world café format to exchange perspectives and requirements for production routes in terms of a circular economy.
The CARBAFIN team of acib and Graz, University of Technology has published a paper on continuous bioprocessing of glucosides from sucrose using the enzyme sucrose-phosphorylase. The advantage of this continuous approach is the tight interconnection of production (upstream) and purification (downstream) process steps as well as a stable operation at excellent output parameters over weeks. A crucial part of the technology is the catalyst immobilization by whole cell encapsulation in porous polymer materials. In other words, a kind of a “nanobioreactor” is created to produce the desired glucoside more efficiently.
The process paves the way for an intensified glucoside production at industrial scale and shows considerable replication potential for other glycosylation products derived from sucrose.
Read the full article and access related data sets.
It’s getting “hot in the city” but this is no problem for the thermostable sucrose phosphorylase which was engineered by our CARBAFIN team at University of Ghent. The biocatalyst derived from Bifidobacterium adolescentis can cope with temperatures higher than 50°C, which offers a reduction of microbial contamination during the production process of glucosylglycerol, a moisturizing ingredient in skin care products. The engineered enzyme shows a significantly improved efficiency, and therefore, gained relevance for cosmetic or food industry. For more details check out the original publication, published in ChemBioChem in May 2021.
Franceus J, Ubiparip Z, Beerens K, Desmet T: Engineering of a thermostable biocatalyst for the synthesis of 2-O-Glucosylglycerol. 2021, ChemBioChem.
The CARBAFIN technology offers a broad spectrum of applications. Quite recently, the team of Graz University of Technology was successful in applying two of the CARBAFIN enzymes, cellobiose phosphorylase and cellodextrin phosphorylase for the bottom-up synthesis of reducing end thiol-labeled cellulose material in a cascade reaction. This is important for a controlled assembly of functional nano-composites and promotes applications for example in electrochemistry, biosensing, catalysis or medicine.
The original article was published in open access journal “Carbohydrate Polymers”.
Zhong C, Zajki-Zechmeister K, Nidetzky B: Reducing end thiol-modified nanocellulose: Bottom-up enzymatic synthesis and use for templated assembly of silver nanoparticles into biocidal composite material. 2021, Carbohydrate Polymers, Vol 260.
Our team from Ghent University has released a Mini-Review presenting an overview of the application of β-glucan phosphorylases in carbohydrate synthesis. Derived carbohydrates (β-glucans) such as cellodextrins can be used as ingredients and additives in the food, feed or cosmetic industry. There is no doubt that the relevance of this enzyme class is increasing for these industrial sectors and will warrant spending more efforts on enzyme engineering, as was also shown in the CARBAFIN project.
Have a look on the publication by following the link:
Ubiparip, Z., De Doncker, M., Beerens, K., Franceus, J., Desmet, T. β-Glucan phosphorylases in carbohydrate synthesis. Appl Microbiol Biotechnol (2021). https://doi.org/10.1007/s00253-021-11320-z
CARBAFIN has released a review about cellodextrin phosphorylase and its suitability for a bottom-up production of cellulose derivatives. So far, these derivatives are produced top-down from lignocellulosic substrates. However, for some product classes a more controllable and “property-tunable” approach would be clearly preferred (eg hydrogels). The publication explores the characteristics of the enzyme and the advantages of an enzymatic production of soluble cello-oligosaccharide.
Link to orginal publication
The optimization of chemical reactions always implies to know the underlaying kinetics like the back of one’s hand. This becomes even more important if a reaction is going to be applied for commercial-scale production! Sucrose phosphorylase, the central enzyme of CARBAFIN, was characterized in terms of kinetics related to the donor substrate: for the glycosylation of glycerol, the effect of donor substrates, in particular sucrose and glucose-1-phosphate was explored.
The results, published by CARBAFIN partner Graz University of Technology, give an explanation for the kinetic behavior of sucrose phosphorylase and show that type and concentration of the glucosyl donor have a significant impact on enzyme selectivity for product formation.